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  • Home
  • Raman
    • BioProcessing
    • Micro Analysis >
      • RXN1 microprobe
      • Raman WorkStation
    • Macro / PAT >
      • PhAT
      • Raman WorkStation
    • Reaction Monitoring >
      • RXN1
      • RXN2 multi
      • RXN2 Hybrid
      • RXN3 Pilot
      • RXN4
    • HandHeld >
      • Serstech 100
      • NanoRam
      • TacticID
    • Portable >
      • i-Raman Plus
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      • i-Raman Pro-ST
      • i-Raman EX
      • GemRam
    • Gas Analysis >
      • RXN4 Gas-Phase Analyzer
  • FTIR
    • ThreatID - Portable FTIR
  • Peptide 합성
    • 펩타이드 자동합성기 >
      • PurePep Chorus
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      • Prelude X
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    • 아미노산
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      • EZ-PICK III
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      • Micro TouchPick Pen
      • Microscope Slices
      • Sample Holders
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      • SurveyIR - Micro Analysis
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Home > Peptide Synthesizer > Prelude X

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Prelude® X
Faster synthesis of simple and complex peptides with higher purity

Introducing the newest peptide synthesizer from Protein Technologies: the Prelude X. Building upon the strength of the original Prelude platform, the Prelude X adds heating, oscillation mixing, and UV monitoring to deliver uncompromised speed, yield, reagent savings, and flexibility, creating the most complete peptide synthesis solution available. Heating conditions in each of the 6 reaction vessels can be independently set to enable flexible, customized reactions. Real time UV monitoring with available Single-Shot amino acid delivery ensures that reactions are completed efficiently and no excess reagent is wasted. Combined with automatic cleavage and flexible pre-activation options, the Prelude X enables researchers to synthesize routine and difficult peptides with unparalleled speed and efficiency.

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Feature

Rapid Induction Heating
The Prelude® X is the first instrument of its kind to include rapid induction heating. The patent-pending technology takes just seconds to reach temperature, and enables controlled heating between 25 degrees C and 90 degrees C. Each of the 6 reaction vessels is equipped, and the heating parameters can be independently programmed and controlled. The technology is fully-compatible with oscillation mixing, which ensures even temperature profiles, homogenous chemistry, and high yields.

Oscillation Mixing
All 6 reaction vessels on the Prelude X are equipped with oscillation mixing. This method creates a uniform heating profile throughout the reaction vessel, eliminating the potential for hot spots or burnt resins. Additionally, it functions to keep resins off the side walls and submerged within the solution. Combined with the fully adjustable nitrogen mixing that is also included on each reaction vessel, the Prelude X allows complete control of reaction parameters for precise and reproducible peptide synthesis.

Real-Time UV-Monitoring
Minimize trial-and-error when synthesizing difficult peptides using real-time UV monitoring on the Prelude X. The Prelude X monitors the reaction solution while it’s mixing – enabling full control of the deprotection reaction times, not just the number of repetitions.

Efficient Reagent Additions
The Prelude X comes with up to 27 amino acid positions to accommodate any chemistry. Expensive reagents can be added to any reactor without priming or wasting a drop using Single-Shot™ deliveries, thus saving money with no manual intervention required and allowing greater automation capacity.

Automated Cleavage
The Prelude X features automated, fully customizable cleavage options. Choose to cleave immediately (Fmoc only), at the end of a synthesis, or at a specified date and time. Cleaved peptides are collected in convenient 50 mL centrifuge vials. All PTI synthesizers are built with robust TFA-resistant materials, so you can cleave for years on the instruments without worry.

Preactivation
The Prelude X has the ability to run 3 preactivation reactions in parallel. This is ideal for sterically hindered amino acid additions. Additionally DIC/HOBt chemistry can be optimized and go straight to scale-up.

Top-Down Washing
Efficiently rinses your reaction between steps.

Reliability – the PTI difference
Other systems use off-the-shelf valves that leak, cross-contaminate, and quickly fail when exposed to harsh peptide synthesis chemicals. Our patented valve block system was specifically designed for peptide synthesis, so you get zero dead volumes, no cross-contamination, and worry-free performance that lasts for years.

Personalized User Databases
Individual users can create their own programs, peptide sequences, and syntheses that can only be accessed from their own personalized user database. Individual databases can be stored on the instrument, or on a portable USB drive.

E-mail Notification Feature
Have the Prelude X send an email to a computer or cell phone at a specified program step, at the beginning of each cycle, or when an error occurs.

Safe Response Feature
The Prelude X can rinse and drain a reaction with a solvent of choice if a synthesis is interrupted for any reason.

Dynamic Sequence Programming
Need more flexibility? The Dynamic Sequence Programming option allows the reaction vessels to operate semi-independently. Have one peptide run a special cycle in the middle of a sequence while the other reaction vessels sit idle. Add pre- or post-processing cycles. Or add or remove amino acids from a peptide sequence.


Specification

Number of reaction vessels
Synthesis scale range

Reaction vessel volume
 

Number of solvent positions/sizes
 
Number of amino acid positions

 


Chemistries supported
Activation
Throughput
Fluid measurement technique
 
Fluid transfer method

 
Agitation method
 
 
Optional heating method

Cleavage
Waste container

Control interface

 
Reporting

Power
Dimensions
Weight

Warranty

Optional


6 (3 with pre-activation option)
0.005 - 1 mmol per reaction vessel

Plastic, disposable - 10 mL, 45 mL sizes
Borosilicate glass - 10 mL, 40 mL

1 primary solvent, 8 L capacity
7 user-defined solvent positions, 1 - 4L & 6 - 1L capacities

27 amino acid bottles
10 mL, 120 mL, and 400 mL sizes
Pre-packaged and empty bottles available
Single-Shot™ delivery option for specialty reagents

Fmoc, t-Boc, organic, peptoid, combinatorial, branched, PNA
HBTU, HATU, HCTU, TBTU, PyBOP, others
User defined
Selectable fixed loop measurement with optical sensors
 for amino acids and reagents, timed delivery of solvents
Positive pressure with nitrogen
Top-down washing
 
Nitrogen bubbling - adjustable, and or optional
Oscillation mixing - adjustable

Induction heating with IR pyrometer temperature sensing

Automated on-board cleavage, separate fluid path and vent
(1) 20 L D.O.T rated container with over-flow sensor in cap

Proprietary Prelude X control software, internal CPU, external
keyboard, mouse, flat panel monitor
 
Real-time log of each instrument function, print to file or printer

115 VAC or 230 VAC, 60 Hz or 50 Hz single phase
34.5”W, 28”D, 36.25”H (87.6 cm W, 71 cm D, 92 cm H)
Approximately 260 lbs. (118 kg)

One year, parts and labor

Dynamic Sequence Programming (Cat#: PPX-DYN-SEQ-PGM)
E-mail-Text Message Status Reporting (Cat#: PPX-TXT-EML-RPT)
UV Monitoring (Cat#: PPX-UV-OPT)
Induction Heating (Cat#: PPX-IHEAT-OPT)
Pre-activation Programming (Cat#: PPX-PRE-ACT-PGM)
Oscillation Mixing (Cat#: PPX-SHAKE-OPT)
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