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BioProcessing

One In Situ Probe, Multiple Continuous Real-time Measurements of the Bioprocess
The RAMANRXN SYSTEMS™ suite of analyzers has set the standard for analyzing, monitoring, and controlling bioprocesses since its introduction in 2009. Raman spectroscopy provides the chemical specificity of a mid-IR analyzer with the ease of sampling of a near-IR analyzer.
The RAMANRXN2™ analyzer is ideally suited for applications in Development scale (10ml – 20L), Pilot scale (50L-500L), and Manufacturing scale (500L – 15,000L+) bioreactors. The ability to scale with the bioprocess enables these analyzers to monitor, measure, and optimize the bioprocess throughout its life cycle. The multi-channel RAMANRXN2™ enables the use of a single-analyzer in support of up to 4 probes, simultaneously utilized in as many bioprocesses. The RAMANRXN2™ 4-channel Raman analyzer operates sequentially allowing both fast analysis per channel and programmable channel integration.
Improve Process Performance
  • Enables Real-time Continuous Measurement and Control of the Bioprocess
Reduce Operating Costs
  • Eliminate Costly Consumables and Labor Intensive Sampling Practices
Increased Repeatability & Reliability
  • Mitigates Concerns about Excessive Downtime and Instrument to Instrument Variability
Proven Technology for Bioprocess Applications
  • Successfully Utilized in Bioprocessing Applications by End-users  Since 2009
Scalability from PD to Pilot and into GMP Manufacturing
  • Ensures Continuity Throughout the Product Life Cycle
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Single-use Raman Probes (SUB Compatible)

Kaiser's new single-use Raman probes enable our proven PAT bioprocessing solutions for single-use bioreactors (SUB). SUB platforms are commonly used for biopharmaceutical production due to the benefits of flexibility, sterility, and reduced cleaning burden.
Kaiser's SUB-Optic System consists of a disposable SUB fitting that is compatible with most SUB platforms and was developed and tested according to industry standards for single-use sensors.
Data generated from Kaiser Raman SUB-Optic Systems are identical to data from our reusable probes (bIO-LAB, bIO-PRO), allowing easy transfer of multi-attribute Raman methods across various bioreactor types and scales from PD to cGMP.
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bIO-LAB/PRO for Bioprocess Applications

The bIO-LAB Probe reflects the coupling of the flexible RAMANRXN™ Probe head with either the bIO-Optic-120 or bIO-Optic-220, or bIO-Optic-320, or bIO-Optic-420 to enable in situ measurement of the bioprocess, and suitability for autoclaving and/or gamma sterilization. The design of these probes, complete with their industry standard 120mm, 220mm, 320mm, or 420mm lengths and 12mm OD, make them ideally suited for both glass and plastic benchtop bioreactor/fermentor applications requiring headplate entry. Additionally, their Pg 13.5 threading enables their use with industry leading benchtop bioreactor manufacturers.
The fixed focus design of the bIO-Optic provides long-term measurement stability along with superior signal performance which is essential for transferable, high performance Raman-based bioprocess analysis.

Probe Features
  • 12mm OD
  • 120mm(bIO-Pro), 220mm, 320mm, or 420mm Lengths
  • Right Angle Connection(bIO-Pro)
  • Autoclavable or Gamma Sterilizable
  • Pg 13.5 Threaded Connector
  • One In / One Out Fiber Optics
  • Integrated "Laser On" Indicator
  • Patented Probe Design
  • Compatible with Industry Leading Benchtop Bioreactors(bIO-Lab)
  • Compatible with Industry Standard Housings
    for 25mm Side Ports (bIO-Pro)
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bIO-LAP
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bIO-Pro

BioPAT® Spectro  A Quality by Design Tool That Unlocks the Full Potential of Raman Spectroscopy

Automated Glucose Control in Ambr® Bioreactors Using Raman Spectroscopy​
  • Automated integration of Raman spectroscopy to Ambr® is now possible
  • Fully automated data acquisition and alignment saves a significant amount of user time
  • A high quality glucose model was generated covering a wide concentration range (up to 15 g/L)
  • Ambr® systems enable robust model building due to a range of setpoints and spiking of samples
  • Cell cultures controlled by integrated Raman or Nova FLEX2 analyzers showed very comparable glucose and VCD profiles

​Glucose Control Results
  • Glucose was maintained in culture in the range of 1-8 g/L (glucose readings >5 g/L were due to glucose in feeds) (Fig. 6)
  • Integrated Raman and Nova FLEX2 glucose assays were in very close agreement throughout (Fig. 6).
  • Cell culture profiles (Fig. 7) were very similar for glucose control based on Raman or Nova FLEX2 assays
  • BioPAT® Spectro in Ambr® enables fully automated glucose control capabilities similar to other analytical techniques (Fig. 7)
  • Further work will include confirmation of the Ambr glucose model performance in Sartorius’ Biostat STR bioreactors(50-2000 L scale)