Ultra PurePep Pathway “Inside”
The proprietary ultra PurePep™ Pathway is enhanced with a dedicated microfluidic matrix valve block pathway for each reaction vessel resulting in pure peptides. Nitrogen and vacuum move all fluids through the system, the membranes are resistant to harsh chemicals, there is zero dead volumes, no cross-contamination, and best-in-class performance that lasts for years. Other systems use off-the-shelf valves that leak or corrode, result in cross-contamination, and quickly fail when exposed to harsh peptide synthesis chemicals. With the ultra PurePep Pathway inside the Tribute®instrument, all fluid deliveries are verified via optical sensors and you have reliable peptide purity every time.
Infrared (IR) heating
Efficient heating, reaching set temperature in minimal time. It is also fully-compatible with vortex mixing, so you can be sure you have an even temperature profile. Other heating systems use nitrogen mixing, which may result in hot and cold spots and effect the consistency of your chemistry.
Real-Time UV monitoring
Minimize trial-and-error when synthesizing difficult peptides using the Intellisynth® real-time UV monitoring on the Tribute instrument. What makes our UV monitoring different? Other systems just monitor the waste stream. We monitor your reaction solution while it’s mixing – that’s why we’re the only ones who can control the deprotection reaction times, not just the number of repetitions. Real-time UV monitoring ensures that reactions are completed efficiently and no excess reagent is wasted.
Tribute can deliver TFA to the reaction vessel from a solvent bottle or an amino acid vial. Afterwards, your cleaved peptide is collected in a 50 mL centrifuge vial in Tribute´s convenient collection stand. Just add either directly to the collection vial and centrifuge to precipitate your peptide!
Automated cycle dependent volume incrementation
Tribute can automatically adjust delivery volumes to account for resin volume increases as your synthesis progresses. Delivery volumes will increase by an increment of your choice each cycle.
Number of reaction vessels
Synthesis scale range
Reaction vessel volume
Number of solvent positions/sizes
Number of amino acid positions
Fluid measurement technique
Fluid transfer method
2 and no resin loader required
0.005 - 2.0 mmol (up to 2.1 g of resin per reaction vessel)
Plastic, disposable: 10 mL, 45 mL
Borosilicate glass: 10 mL, 40 mL
5 pressurized solvents (1 to 4 L each)
101 position amino acid rack, barcode scanning of amino acid vials
Fmoc, t-Boc, organic, peptoid, combinatorial, branched, PNA
In situ or pre-activation with HBTU, HATU, HCTU, TBTU, PyBOP, PyOxim, DIC/OxymaPure, DIC/HOBt, DIC/Oxyma-B and others
Fixed loop measurement with optical sensors
Nitrogen or Argon pressure. Top down or bottom-up deliveries
Nitrogen bubbling and vortex mixing - adjustable
One 20 L container with over-flow sensor
Proprietary Tribute control software, internal CPU, full-color chemical-resistant touchscreen
Real-time log of each instrument function, print to file or printer
115 V/60 Hz or 230 V/50 Hz
43” x 25.4" x 23.2” (109 cm x 64 cm x 59 cm)
Approximately 100 lbs. (45 kg)
One year, parts and labor.
Automated cleavage (Optional, Fmoc only, Cat#: TPS-CLV-OPT)
Infrared (IR) Heating (Cat#: TPS-IR-OPT)
Intellisynth Real-time UV Monitoring and feedback control system and software (Cat#: TPS-UV-OPT)
E-mail-Text Message Status Reporting (Cat#: TPS-TXT-EML-RPT)
User Workstation Utility